Journal:

Article Title: Problems Related to Determination of MICs of Oximino-Type Expanded-Spectrum Cephems for Proteus vulgaris

doi:

Figure Lengend Snippet: Comparative susceptibilities of 42 clinical isolates of P. vulgaris to expanded-spectrum cephems, as determined by broth microdilution and agar dilution methods

Article Snippet: The values of V max , K m , and K cat of purified P. vulgaris β-lactamase (Wako Pure Chemicals Industries, Ltd., Osaka, Japan) were determined by computerized spectrophotometry using various absorbances at appropriate wavelengths for CER, CTX, CEM, CPD, ZOX, MOX, and CTB.

Techniques: Incubation

Journal:

Article Title: Problems Related to Determination of MICs of Oximino-Type Expanded-Spectrum Cephems for Proteus vulgaris

doi:

Figure Lengend Snippet: Comparative susceptibilities of P. vulgaris B317 and other strains to various antibiotics, as determined by broth microdilution and agar dilution methods

Article Snippet: The values of V max , K m , and K cat of purified P. vulgaris β-lactamase (Wako Pure Chemicals Industries, Ltd., Osaka, Japan) were determined by computerized spectrophotometry using various absorbances at appropriate wavelengths for CER, CTX, CEM, CPD, ZOX, MOX, and CTB.

Techniques: Mutagenesis

Journal:

Article Title: Problems Related to Determination of MICs of Oximino-Type Expanded-Spectrum Cephems for Proteus vulgaris

doi:

Figure Lengend Snippet: Results of determination of MICs of CTX (a to c) and AMP (d to f) against P. vulgaris B317R/pMD501 by the broth microdilution method. Reading of the MIC was done after 48 h of incubation.

Article Snippet: The values of V max , K m , and K cat of purified P. vulgaris β-lactamase (Wako Pure Chemicals Industries, Ltd., Osaka, Japan) were determined by computerized spectrophotometry using various absorbances at appropriate wavelengths for CER, CTX, CEM, CPD, ZOX, MOX, and CTB.

Techniques: Incubation

Journal:

Article Title: Problems Related to Determination of MICs of Oximino-Type Expanded-Spectrum Cephems for Proteus vulgaris

doi:

Figure Lengend Snippet: Effect of agar concentrations on susceptibility testing of P. vulgaris against CTX

Article Snippet: The values of V max , K m , and K cat of purified P. vulgaris β-lactamase (Wako Pure Chemicals Industries, Ltd., Osaka, Japan) were determined by computerized spectrophotometry using various absorbances at appropriate wavelengths for CER, CTX, CEM, CPD, ZOX, MOX, and CTB.

Techniques:

Journal:

Article Title: Problems Related to Determination of MICs of Oximino-Type Expanded-Spectrum Cephems for Proteus vulgaris

doi:

Figure Lengend Snippet: Expression of β-lactamase activity in P. vulgaris B317 and other strains under noninducing and inducing conditions

Article Snippet: The values of V max , K m , and K cat of purified P. vulgaris β-lactamase (Wako Pure Chemicals Industries, Ltd., Osaka, Japan) were determined by computerized spectrophotometry using various absorbances at appropriate wavelengths for CER, CTX, CEM, CPD, ZOX, MOX, and CTB.

Techniques: Expressing, Activity Assay, Plasmid Preparation, Mutagenesis

Journal: Frontiers in Microbiology

Article Title: Antibacterial Activity of Stenotrophomonas maltophilia Endolysin P28 against both Gram-positive and Gram-negative Bacteria

doi: 10.3389/fmicb.2015.01299

Figure Lengend Snippet: Antimicrobial activity of endolysin P28 against various species.

Article Snippet: Proteus vulgaris , CCTCC AB91103 , 30 , 61.2.

Techniques: Activity Assay

Journal: Neurobiology of disease

Article Title: Combined chondroitinase and KLF7 expression reduce net retraction of sensory and CST axons from sites of spinal injury

doi: 10.1016/j.nbd.2016.12.010

Figure Lengend Snippet: (A) shows the design of plasmid DNA used for the production of Lenti-Chase, indicating mutations to enhance thermal stability and to block aberrant glycosylation. (B) 293T cells were transfected with plasmid encoding EBFP control or chondroitinase, or transduced with Lenti-chase, and the conditioned media tested for chondroitinase activity by colorimetric DMMB assay. Compared to a standard curve of chondroitinase activity (grey circles), media from EBFP-transfected cells showed <0.1U/ml chondroitinase activity media from cells transfected or virally transduced to express chondroitinase showed >7 U/μl.

Article Snippet: Cloning of KLF7 and Lenti-Chondroitinase DNA encoding P. vulgaris chondroitinase ABC (accession number {"type":"entrez-protein","attrs":{"text":"AAB43331","term_id":"1828877","term_text":"AAB43331"}} AAB43331 ) with a 5′ signal sequence from matrix metalloprotease two (MMP2, accession NM008610) and codon optimized for mammalian expression was synthesized by Genscript.

Techniques: Plasmid Preparation, Blocking Assay, Glycoproteomics, Transfection, Control, Transduction, Activity Assay, Dimethylmethylene Blue Assay